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CRISPR / Cas9
   
 
CRISPR Stable Knockout Cell Line Generation
 

 

Our Basic Package (C208) Includes Deliverables Lead Time
  1. Select from any of the listed cell lines (pre-determined easy to transfect cell lines)*
  2. sgRNA vector design, construction & delivery into cells
  3. Clonal screening and selection
  4. Screening & validation of knockout by Sanger sequencing**
  5. Expansion & cryopreservation of selected clone(s)
  6. PCR based Mycoplasma testing
Sequence verified knockouts (at least 1 clone, 2 vials per clone)**, mycoplasma tested with a service report. 12-16 weeks^^

Add-On Services^

Quantity

Cat. No.

Cell Line of Your Choice for Gene Editing#

1 Cell Line

C141

WT Control Cell Line Expressing Cas9 for Comparison
(50% discount for academic customers if ordered with the basic package)

1 Cell Line

C142

Additional Clones

1 Clone

C143

Additional Vials of Delivered Clones

1 Vial

C144

Validation Service by Western Blot (Up to 10 Clones)##

1 Service

C145

Off-Target Analysis by Whole Genome Sequencing

Per Clone

C146

Additional rounds of selection and screening by Sanger Sequencing

1 Screening

C147

STR Profiling of WT and Knock-out
(50% discount if ordered with the basic package)

1 Service

C287

 

      
 


Additional Information


* Choose from HEK293, HEK293T, A549, HeLa, MDCK, A375, HepG2, HT1080 or U87MG. These cell lines are available as Cas-9 expressing versions or parental version. Cas9 expressing version will be used by default.If the parental version is preferred, please request prior to order placement.

**This pricing is valid for a single gene knockout for diploid loci only.

#Customer is required to provide atleast 2 million cells, 1L of propagation media/any special coated flasks if needed (if cells need DMEM or RPMI, ABM will supply media). Also, the cell line must tolerate single cell cloning and display adequate transfection and transduction efficiency.

##Pre-validated antibody needs to be provided by customer with appropriate positive control (preferably with supporting data).

^All “add on” services need to be indicated prior to order placement as the project design will need to be finalized prior to start of the service.

^^ Final Lead time may vary depending on actual growth rate of cell line while expanding from single cell.
 
 
 
 

Workflow


The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system offers cutting edge RNA-Guided endonuclease technology for highly specific and customizable genome editing. With the use of a target sequence specific guide RNA, as well as the highly specific and programmable CRISPR associated nuclease (Cas9), abm offers an efficient and targeted genome editing service with minimal off-target effects.



You determine the gene of interest for knockout in the desired cell line and we can do the rest.The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system offers cutting edge RNA-Guided endonuclease technology for highly specific and customizable genome editing. With the use of a target sequence specific guide RNA, as well as the highly specific and programmable CRISPR associated nuclease (Cas9), abm offers an efficient and targeted genome editing service with minimal off-target effects.




You determine the gene of interest for knockout in the desired cell line and we can do the rest.
 
 
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