Designed as an easy, effective way to verify your
genomic editing process.
CRISPR-edited samples are used as a template in PCR reactions
targeting your specific region of interest. The products are then denatured and
re-annealed to produce mismatches within the double strand. Our detection enzyme
is able to recognize such mismatches and cleaves the strands to produce band
sizes that are easily distinguishable upon gel analysis.
CRISPR Genomic Cleavage Detection Kit contains all the necessary reagents to
detect genomic cleavage. The protocol has a rapid 4 hour processing time and is
completed in a single tube. This quantitative, ready-to-use assay will be a
great addition to any genomic-editing toolbox.