The pCambia vector backbone is derived from the pPZP vectors.
The pCambia1300vector offers:
-high copy number in E.coli for high DNA yields
-pVS1 replicon for high stability in Agrobacterium
-restriction sites for modular plasmid modifications and small but adequate polylinkers for introducing DNA of interest
-bacterial selection with kanamycin
-plant selection with hygromycin B
Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal.
This vector contains minimal heterologous sequences for plant transformation and selection of transformants, allowing insertion of desired genes for transformation into plants but require all promoter and terminator sequences for plant expression of newly cloned genes.
The pUC18 polylinker within the lacZa fragment allows blue/white screening of clones in E. coli cloning work.