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이츠바이오  Homepage Email [2012-08-29 11:05:30]  HIT : 5827  

   ABM (Applied Biological Materials Inc.)

   For siRNA

  iLenti™ siRNA Expression System

ProductIndividual
Cat. No.
QuantityKit Catalog Number
LV300LV301LV310LV311LV098LV099
Linear iLenti™ Vector LV014 25ul V   V      
Linear iLenti™ GFP Vector LV016 25ul   V   V    
Lenti-Packaging Mix LV003 100ug V V V V V  
Lentifectin™ G074 1.0ml V V     V  
293T Cells LV010 1x106 V V       V
Lenti-EGFP LV011-a 10ug V V       V
iLenti Sequencing Primers LV012 100ul V V V V    

 

Description
  Recombinant lentiviral vectors have been shown to be a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo. Through years of experience with lentiviral vectors, scientists at ABM have developed our own proprietary Lentiviral expression vectors and pLenti-combo packaging mix that allow for rapid production of recombinant lentiviral vectors with titers up to 107IU/ml. iLenti™ RNAi Expression System allows for the efficient expression of any target siRNA through transfection or lentiviral infection of target cells. The system is based on an unique convergent promoter design for greater efficiency of target gene knockdown without the need of a hair-pin loop structure design commonly required for a single promoter vector.
 

Advantages of ABM's iLenti RNAi Fusion Expression Vectors

  • stable plasmid; rare plasmid DNA re-arrangement
  • very high yielding plasmid
  • no special competent cells required
  • convergent promoter design
  • higher efficiency of target gene knockdown
  • ABM is THE source for your lentiviral expression needs!

 

 Vectors

iLenti™ Vector iLenti™-GFP Vector
Click for Details Click for Details

 

 User Manual     :                Infection Protocol     :

 

Related Products

Cat. No.

  Product

Selection Marker

LV006

  GFP Control Lentivirus (10ml)

Neomycin

LV007

  β-Gal Control Lentivirus (10ml)

Neomycin

LV011-b

  β-Gal Control Lentivector (10ug)

N/A

LV023

  iLentiTM-GFP Negative Control Lentivirus (10ml)

Puromycin

 

  Custom iLenti™ siRNA Service

Service

  Description

Cat. No.

iLenti™ siRNA Cloning

  1 iLenti™ Construct, 500ng

C043

  1 iLenti™-GFP Construct, 500ng

C043-G

  4 iLenti™ Constructs, 4 x 500ng

C044

  4 iLenti™-GFP Constructs, 4 x 500ng

C044-G

  Negative Control Scramble Vector, 500ng

LV015

  Negative Control Scramble Vector with GFP, 500ng

LV015-G

Custom Lentivirus Production

  Custom Recombinant Lentivirus (106IU/ml; 10ml)

LV002

  High-Titer Custom Recombinant Lentivirus (107IU/ml; 1ml)

LV002-a

  High-Titer Custom Recombinant Lentivirus (108IU/ml; 550ul)

LV002-b

  High-Titer Custom Recombinant Lentivirus (109IU/ml; 100ul)

LV002-c

  High-Titer Custom Recombinant Lentivirus (1010IU/ml; 100ul)

LV002-d

 

Description

Custom-made, ready-to-use iLenti™ and iLenti™-GFP siRNA for transfection or infection.

  • A single construct
  • 4 constructs
  • Guaranteed knockdown for any gene
  • Every construct with a GFP reporter
  • Less than the material cost, why do it yourself?


Simply provide us with your gene's accession number for any target gene or siRNA target sequence and we will create the lentivirus siRNA construct for you within 2 weeks!

Once we get your gene name, we will search our siRNA database of over 20,000 publications covering over 7,000 human and mouse genes. Then we will design four constructs with high knockdown efficiency against the target. If your gene of interest is not yet published, we will design four target sequences using our internal siRNA software. With four siRNA constructs, at least one of the constructs should give over 70% gene knockdown efficiency in appropriate target cells with over 70% transfection efficiency.

We also have a scramble construct in our proprietary vector as the ideal control for your experiments.

 

Advantages of iLenti™ siRNA:

  1. siRNA in lentiviral vectors for either transfection or viral infection.
  2. iLenti™ vectors are very stable, no special competent cells are required.
  3. iLenti™ uses more potent 27-29 bp oligos.
  4. iLenti™ uses convergent promoters to avoid hairpin loop structures, making sequencing and plasmid growth much easier.

 

 

  siRNA Lentivector

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              http://www.abmgood.com/siRNA-shRNA-RNAi-Lentivectors-Lentiviruses.html

 

 

 

 

(2015-03-31)
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