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New! 5X All-In-One RT MasterMix 프린트 
이츠바이오  Homepage Email [2013-07-01 09:23:29]  HIT : 4985  
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   ABM (Applied Biological Materials Inc.)

 

 5X All-In-One RT MasterMix

 

Our 5X All-In-One RT MasterMix is a ready to use mixture of all the reagents required for first-strand cDNA synthesis, provided in a 5X concentration.

This optimized reaction mastermix contains our EasyScript™ RTase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a balanced concentration of Oligo(dT) and Random Primers, all you need to add is your RNA template and nuclease-free H2O.
RNaseOFF Ribonuclease Inhibitor effectively protects RNA template from degradation. Oligo(dT) anneals selectively to the Poly(A) tail of mRNAs. Random Primers do not require the presence of Poly(A) and they are utilized for the transcription of mRNA 5’-end regions. The first-strand cDNA product can then be used directly as a template in PCR and qPCR.

 

  5X All-In-One RT MasterMix

  Components

G485

G486

  5X All-In-One RT MasterMix

50 μl

200 μl

  Nuclease-free H2O

1 ml

1 ml

  Size

25 rxns

 100 rxns

 

 

 

  Next Generation Reverse Transcriptases

 

 High Accuracy from Fidelity-Enhancing Subunit

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Figure 1: EasyScript™ RTase series Accuracy Rate
EasyScript™ RTases series have the highest accuracy rate compared to leading competitors.   Accuracy rate = 1/error rate.

Native RTases have notoriously low proofreading ability but abm has circumvented this problem with its new EasyScript™ and EasyScript Plus™, which have the highest accuracy rates when compared with other commercial RTases. Both EasyScript™ and EasyScript Plus™ contain a unique fidelity-enhancing subunit which drastically enhances accuracy in reverse transcription, making abm’s RTases the most reliable on the market (Figure 1).

 

 High Yield and Sensitivity

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Figure 2: Sensitivity of EasyScript™ Reverse Transcriptase
PCR amplification using human cDNA synthesized with EasyScript™ with varying amounts of starting RNA ( 2000 pg - 0 pg), followed by electrophoresis on a 1% agarose gel.

 Template sensitivity is crucial for reverse transcription reactions and is paramount to the usability and success of an RTase application. EasyScript™ and EasyScript Plus™ have exceptional template sensitivity that ensures high yields of full length cDNA synthesis, even in cases of very low amounts of starting RNA template. These RTases have been strategically engineered to synthesize appreciable amounts of full-length single-stranded cDNA with as little as 0.1 pg of total starting RNA, as demonstrated in Figure 2.

 

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 First Strand cDNA Synthesis Yield

Figure 3: Data provided by a leading whole genome institure - BC Genome Science Center.
Result : Next Generation Enzyme design (abm’s and Fisher’s) shows superior performance over first generation enzymes as assayed by Qubit.

 

 

 Next Generation Reverse Transcriptases

 

  5X All-In-One RT MasterMix

  Components

G485

G486

  5X All-In-One RT MasterMix

50 μl

200 μl

  Nuclease-free H2O

1 ml

1 ml

  Size

25 rxns

 100 rxns

 

 Reverse Transcriptases

G231

EasyScript RTase   (200U/ul)                                         5,000U

G232

EasyScript RTase   (200U/ul)                                       20,000U

G177

EasyScript Plus RTase   (200U/ul)                                5,000U

G237

EasyScript Plus RTase   (200U/ul)                              20,000U

 

 FirstStrand cDNA Synthesis Kit

G233

EasyScript cDNA Synthesis Kit                                   25 rxns

G234

EasyScript cDNA Synthesis Kit                                 100 rxns

G235

EasyScript Plus cDNA Synthesis Kit                          25 rxns

G236

EasyScript Plus cDNA Synthesis Kit                        100 rxns

 

 FirstStrand cDNA Synthesis Supermix

G451

EasyScript cDNA Synthesis Supermix                        25 rxns

G452

EasyScript cDNA Synthesis Supermix                      100 rxns

G453

EasyScript Plus cDNA Synthesis Supermix               25 rxns

G454

EasyScript Plus cDNA Synthesis Supermix             100 rxns

 

 One-Step RT-PCR Kit

G174

EasyScript One-Step RT-PCR Kit                     100 x 50ul rxns

 

 Second Strand cDNA Synthesis Kit

G475

Second Strand cDNA Synthesis kit                             25 rxns

G476

Second Strand cDNA Synthesis kit                           100 rxns

 

 

          RT-PCR 관련 Products brochure

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